Research on special training of doctor-patient communication based on practical teaching in emergency environment / 中华医学教育探索杂志

Objective:To explore whether the special training of doctor-patient communication based on practical teaching can develop undergraduates communication skills in emergency environment.Methods:A total of 60 medical undergraduate students of grade three were enrolled randomly and were divided equally into the control group and the training group, with 30 ones in each group. The students in control group were taught with basic theory of doctor-patient communication and required to take part in peer role-playing. Besides the same content of the courses, the students in training group were additionally given case discussion on internet medical care platform and clinical clerkship. Before and after training, self-assessment scale and Maastricht comprehensive evaluation scale were used to evaluate the doctor-patient communication ability of medical students. SPSS 22.0 software was used to analyze the reliability of the scale and ANOVA were adopted to analysis the data.Results:There was no significant difference in communicative skills between the two groups before training. The students of the control group exhibited no significant increase of communicative skills after training. In a contrast, the self-assessments completed by the students in training group improved significantly after the training. The expert-rating communication skills including such overall communication ability and three individual items as exploration, emotion and empathy have improved significantly for the training group after the training. Taking the training courses of doctor-patient communication has a significant impact on overall communication competency for students [ F(time×course)=5.38, P<0.05, Partial η2=0.133)]. Conclusion:The special training of doctor-patient communication with the frame given priority to practice is beneficial to stimulate study interest and improve communication skills for medical undergraduate students. It’s a useful attempt to apply the internet medical care platform to the practice of doctor-patient communication teaching.

In silico design of novel proton-pump inhibitors with reduced adverse effects / 医学前沿

The development of new proton-pump inhibitors (PPIs) with less adverse effects by lowering the pKa values of nitrogen atoms in pyrimidine rings has been previously suggested by our group. In this work, we proposed that new PPIs should have the following features: (1) number of ring II = number of ring I + 1; (2) preferably five, six, or seven-membered heteroatomic ring for stability; and (3) 1 < pKa1 < 4. Six molecular scaffolds based on the aforementioned criteria were constructed, and R groups were extracted from compounds in extensive data sources. A virtual molecule dataset was established, and the pKa values of specific atoms on the molecules in the dataset were calculated to select the molecules with required pKa values. Drug-likeness screening was further conducted to obtain the candidates that significantly reduced the adverse effects of long-term PPI use. This study provided insights and tools for designing targeted molecules in silico that are suitable for practical applications.

Risk factors for multidrug-resistant bacteria recurrent infections in orthopedic patients from 2011 to 2017 / 中华创伤骨科杂志

Objective To explore the clinical characteristics of multidrug-resistant organisms (MDROS) and analyze the risk factors for MDROS recurrent infections in orthopedic in-patients.Methods A retrospective study was conducted of the clinical data of 296 in-patients with MDROS infection from June 2011 to August 2017.They were 216 males and 80 females with an average age of 49.9 years.Their average hospital stay was 37.2 days.Univariate analysis was conducted for items like age,hospital stay,bedridden time,concomitant internal disease (chronic obstructive pulmonary disease,chronic cardiovascular disease and diabetes),open or closed fracture,uninary catheter,use of hormone,stay in ICU,implantation material,incision grade,albumin level,hemoglobin level,reoperation,type of antibiotics and duration of antibiotics use.Multivariate logistic regression analysis was performed using SPSS 20.0 for items with significant differences.Results A total of 352 strains of pathogens were isolated,including methicillin-resistant staphylococcus aureus (26.7％),extended-spectrum β-lactamase-producing Escherichia coli (24.4％) and multidrug-resistant acinetobacter baumannii (24.1％).The pathogens were observed mostly at surgical sites (34.1％) and open wounds (23.0％).Forty-two patients reinfected the same strain after treatment.The multivariate logistic regression analysis revealed the following as the independent risk factors for MDROS recurrent infections in the orthopedic in-patients:hospital stay [OR =4.918,95％ CI (1.642,14.731),P =0.004],long bedridden time [OR=3.583,95％ CI (1.081,11.876),P=0.037],open injury [OR=2.375,95％CI (1.291,4.368),P=0.005],diabetes [OR=6.360,95％ CI (2.112,19.149),P=0.001],and chronic obstructive pulmonary disease [OR=4.170,95％ CI (1.419,12.251),P=0.009].Conclusions To prevent recurrent MDROS infections in orthopedic patients,surgeons should shorten unnecessary hospital stay,encourage ambulation as early as possible,effectively control blood sugar and actively treat concomitant internal diseases in addition to regular use of antibiotics.

The experimental study on repair of rat femoral condyle defect with peripheral blood mesenchymal stem cells combined with absorbable gelatin sponge-self assembling peptide / 实用医学杂志

Objective This study aimed to explore the effect of peripheral blood mesenchymal stem cells combined with porous absorbable gelatin sponge/self assembling peptide composite scaffolds on SD rat femoral con-dyle bone defect reconstruction and provide a new strategy for the repair of bone defects. Methods 30 female SD rats,8W age,were randomly divided into 3 groups,10 every group.The group A was blank control group,group B was porous absorbable gelatin sponge/self assembling peptide composite scaffold group,and group C was periph-eral blood mesenchymal stem cells combined with porous absorbable gelatin sponge/self assembling peptide compos-ite scaffold group. The effect of osteogenesis was observed by paraffin section,hematoxylin eosin staining,X-ray examination,and Micro-CT scanning in 3 dimensional reconstruction of femoral condyle defect. Results Imaging examination showed that the experimental group had better osteogenesis effect. Histological examination showed that a lot of new bone tissue was found in group C,while only a small amount of new bone was found in the group of A and B. Conclusions The experiment shows that peripheral blood mesenchymal stem cells as the seed cells for tissue engineering,combined with porous absorbable gelatin sponge-self assembling peptide has better ability to repair bone defects,and has good application prospect,which is worthy of further research.

Evaluation of peer support education mode for type 2 diabetes control in rural residents / 中华流行病学杂志

Objective To evaluate the intervention effects of peer support education mode for type 2 diabetes control in rural residents.Methods A random cluster sampling method has been used,including 300 rural residents aged above 18 years old from three villages (184 in control group,116 in intervention group),in order to proceed the physical check-up and health education programs.Unchanged rate,transfer rate of patients,rate of impaired glucose tolerance,turn normal rate and other biochemical indicators of patients and people with impaired glucose tolerance from control group and intervention group were analyzed,to evaluate the intervention effects of peer support education mode.Results The glycemic control rate of intervention group for patients and people with impaired glucose tolerance (72.2％ and 71.4％) were higher than control group (43.6％ and 26.7％),but the unchanged rate of intervention group (13.9％ and 0.0％) were lower than control group (42.3％ and 73.3％).Patients with diabetes or glucose intolerance in the education group improved significantly in waist-to-hip ratio,uric acid,total cholesterol and HDL-C.Glycemic hemoglobin level also improved significantly in diabetes patients of the education group.Conclusion Peer support for education intervention seemed beneficial for diabetic control.The combination of education and effect evaluation was important in the evaluation of diabetes prevention and control.Peer support education also benefited the blood glucose control in general population.

Evaluation of peer support education mode for type 2 diabetes control in rural residents / 中华流行病学杂志

Objective To evaluate the intervention effects of peer support education mode for type 2 diabetes control in rural residents.Methods A random cluster sampling method has been used,including 300 rural residents aged above 18 years old from three villages (184 in control group,116 in intervention group),in order to proceed the physical check-up and health education programs.Unchanged rate,transfer rate of patients,rate of impaired glucose tolerance,turn normal rate and other biochemical indicators of patients and people with impaired glucose tolerance from control group and intervention group were analyzed,to evaluate the intervention effects of peer support education mode.Results The glycemic control rate of intervention group for patients and people with impaired glucose tolerance (72.2％ and 71.4％) were higher than control group (43.6％ and 26.7％),but the unchanged rate of intervention group (13.9％ and 0.0％) were lower than control group (42.3％ and 73.3％).Patients with diabetes or glucose intolerance in the education group improved significantly in waist-to-hip ratio,uric acid,total cholesterol and HDL-C.Glycemic hemoglobin level also improved significantly in diabetes patients of the education group.Conclusion Peer support for education intervention seemed beneficial for diabetic control.The combination of education and effect evaluation was important in the evaluation of diabetes prevention and control.Peer support education also benefited the blood glucose control in general population.

Neutrophil to lymphocyte ratio in peripheral blood: a novel independent prognostic factor in patients with head and neck squamous cell carcinoma / 中华肿瘤杂志

Objective@#To investigate whether neutrophil to lymphocyte ratio (NLR) in peripheral blood can be an independent prognostic factor in patients with head and neck squamous cell carcinoma (HNSCC).@*Methods@#Clinical data of 97 HNSCC patients who received surgical treatment in our department between January 2008 and January 2012 were analyzed retrospectively. The 97 patients were divided into low NLR group (NLR≤5, n=69) and high NLR group (NLR>5, n=28) according to the NLR in preoperative peripheral blood. The relationships of NLR and clinicopathological features were analyzed. Kaplan-Meier method was used for univariate survival analysis and Cox proportional hazard model for multivariate survival analysis.@*Results@#The clinical stages were significantly different between high NLR group and low NLR group (P<0.05), however, the age, gender, location, lymph node metastasis, smoking and alcohol of the two groups showed no significant differences (P> 0.05 of all). Univariate survival analysis showed that smoking, lymph node metastasis, clinical stage and NLR value were risk factors for 3-year overall survival (OS) rate and relapse-free survival (RFS) rate of HNSCC patients (P<0.05). The OS rate of high NLR and low NLR groups was 42.9% and 91.3%, and the RFS rate was 44.2% and 80.1%, respectively, with a statistically significant difference (P<0.05 for both). Cox multivariate survival analysis showed that clinical stage and NLR were independent factors for prognostic evaluation of HNSCC patients (P<0.05 for both).@*Conclusions@#NLR level is significantly associated with clinical stage of HNSCC. High NLR is an independent prognostic rick factor and plays an important role in prognostic evaluation of HNSCC patients.

Timing of laparoscopic cholecystectomy in acute pancreatitis with gallbladder stones / 中华肝胆外科杂志

Objective To evaluate the timing,feasibility and necessity of early laparoscopic cholecystectomy (LC) in management of patients with mild to moderate acute pancreatitis with gallbladder stones.Methods The clinical data of 75 patients with mild to moderate acute pancreatitis and gallbladder stones treated from September 2010 to August 2014 in our hospital were analyzed retrospectively.32 patients underwent LC within 48 hours of the pancreatic attack.The results were compared with those from 43 patients with delayed LC.Results All the patients were operated by experienced surgeons.There were no significant differences between the two groups in operation time,postoperative complications,intraoperative blood loss,conversion rates,white blood cell count,percentage of neutrophils and blood amylase before and after the operation (P ＞0.05).Patients who received delayed LC had longer hospital stay [(10.6 ± 1.3) vs (17.1 ± 1.8),P ＜ 0.05].The readmission rate in patients with delayed LC was 30.2％ (13/43),and most admissions occurred within 2 ～4 weeks of hospital discharge.In patients with early LC,one patient developed necrotic pancreatitis and died of septic shock.All the remaining patients were cured.Conclusions It is safe,feasible and necessary to perform LC within 48 h in patients with mild to moderate acute pancreatitis and gallbladder stones.Such patients have a high readmission rate and the best timing of delayed LC is within 2 ～ 4 weeks after discharge from hospital.

Prevalence of hyperuricemia in health check-up population of Beijing suburb / 中华全科医师杂志

Objective To investigate the prevalence of hyperuricemia in health check-up population of Beijing suburb.Methods Total 1 336 rural residents in Nankou Township of Beijing received health check-up from July to Aug 2014,including 686 subjects aged 20-59 years (young/middle-aged group) and 650 subjects aged 60-96 years (elderly group).The blood pressure and body mass index (BMI) were measured;serum uric acid (SUA),fasting blood glucose (FBG) and blood lipids (TG,TC,HDL-C,LDL-C) were determined.The SUA levels ＞ 420 μmol/L for male and ＞ 360 μmol/L for female were defined as hyperuricemia.Results The four quartiles of SUA levels were 27.00-254.59 μmol/L (Q1),254.60-302.35 μmol/L (Q2),302.36-359.78 μmol/L(Q3) and 359.79-702.0 μmol/L (Q4).The prevalence of hyperuricemia was significantly higher in young/middle-aged group than that in elderly group [20.41％ (140/686) vs.13.85％ (90/650),x2 =10.08,P =0.001 5],the systolic blood pressure [SBP,(126.8±15.7) vs.(116.7±12.0)mmHg(1 mmHg=0.133 kPa),t=2.76,P=0.008],FBG [(7.40±4.10) vs.(6.11 ±2.03)mmol/L,t=2.12,P=0.036],TC [(5.52±1.10) vs.(5.23±1.00)mmol/L,t =2.04,P =0.045],LDL-C [(3.5 ±0.7) vs.(2.4 ±0.9)mmol/L,t =2.21,P =0.029]in young/middle-aged group were significantly higher than those in elderly group.BMI,FBG were significantly higher in Q4 than those in other quartiles [BMI:(26.44 ± 3.88) vs.(24.19 ± 3.37),(25.49±3.42) and (25.61 ±3.49)kg/m2,t =2.78,P=0.008;FBG:(8.19 ±1.52) vs.(6.34±1.34),(6.09 ± 1.51) and (6.40 ± 1.98) mmol/L,t =2.80,P =0.007].The triglyceride (TG) levels in group Q3 and Q4 [(1.85 ± 0.90) and (1.92 ± 0.44) mmol/L] were higher than those in Q1 and Q2 [(1.37 ±0.76) and (1.70 ±0.84) mmol/L,t =2.1,P =0.035].Only 9.57％ subjects (22/230)with hyperuricemia was not combined with metabolic disorder;subjects combined with one and two metabolic disorders accounted for 20.87％ (48/230) and 69.57％ (160/230),respectively.Conclusion Screening for hyperuricemia is important for comprehensiye treatment and management of hyperuricemia in rural residents,especially in the young and middle-aged population.

The effect of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres in repairing the rabbits bone defects / 实用医学杂志

Objective To explore the effect of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres in repairing large bone defects of rabbit femoral condyle. Methods Animal models of bone defects were induced in 21 New Zealand white rabbits by drilling holes in bilateral femoral lateral condyles , and the rabbits were equally divided into 3 groups:group A as the control group with the defects untreated , group B treated by filling with nano-hydroxyapatite/collagen scaffolds (NHAC), and group C treated by filling with the nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres (ADM-PLGA-NHAC). At week 12 after implanting , the rabbits were all sacrificed for the implanted scaffolds , which were then examined by X-ray , and Micro-CT 3D reconstruction and in histology for evaluation of the new bone formation. Results X-ray, Micro-CT and the measurement and analysis of BMD indicated thatthere was no significant differencein the new bone formation between group B and group C (P > 0.05). The histological examination revealed that. 12 weeks after operation an evident number of new born bones were seen on the implanted scaffolds in groups B and C , while very few were seen scattering in group A. Conclusion The nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres is effective in repairing bone defect without influencing the prosthetic process.

Study on the antiulcer effects of Veronicastrum axillare on gastric ulcer in rats induced by ethanol based on tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1)

Objective:To assess whether Veronicastrum axillare (V. axillare) can ameliorate ethanol-induced gastric mucosal lesions in rats, reduce the production of pro-inflammatory cytokines, suppress apoptosis and improve local microcirculation disturbances. Methods:Totally 48 male Sprague-Dawley rats were randomly divided into six groups, eight rats in each group. Rats in the normal group and the model group were administered with 0.9%normal saline respectively. Rats in the positive group and ranitidine group were administered with 0.18% ranitidine suspension by intragastric administration respectively. Those in the high dose V. axillare group, the medium dose V. axillare group and the low dose V. axillare group were administrated with V. axillare at the daily dose of 2.8 g/kg, 1.4 g/kg and 0.7 g/kg by intragastric administration. Gastric mucosal lesions were produced by intragastric administration of absolute ethanol. Water extract of V. axillare was successively injected for 14 d and last day was injected 1 h before ethanol administration. Gastric mucosal ulcer index and ulcer inhibitory rate were counted by improved Guth methods. The tissue sections were made for pathological histology analysis. Also, we measured the concentrations of tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1) in gastric mucosal, as an index of the pro-inflammatory cytokines, apoptosis and local microcirculation. Besides, the mRNA contents of TNF-αand ET-1 were measured to verify effects on gene expression by real-time fluorescent quantitative PCR. Results: Water extract of V. axillare significantly ameliorated the gastric mucosal lesions induced by ethanol administration (P Conclusion: Current evidences show water extract of V. axillare is effective for defending against ethanol-induced gastric mucosal lesions, significantly inhibiting the production of pro-inflammatory cytokines and the expressions of TNF-αand ET-1 mRNA, which may be useful for inhibiting apoptosis and improving local microcirculation.

Laparoscopic versus open distal pancreatectomy for pancreatic disease: a meta analysis / 中华肝胆外科杂志

Objective To evaluate the clinical effectiveness of laparoscopic (LDP) versus open distal pancreatectomy (ODP) using meta-analysis.Methods Comprehensive literature search was conducted on articles only in English published from 2006 to 2012 on MEDLINE,EMbase,Cochrane Central Registry of Controlled Trials to compare LDP with ODP for Pancreatic disease.Data were extracted and evaluated by two reviewers independently.The quality of the included trials was evaluated.Meta-analyses were conducted using the Cochrane Collaboration's RevMan 5.1 software.Results Fourteen controlled clinical trials (n=1417) were included.The LDP group was significantly longer than the ODP group in operation time,and was significantly larger in the number of patients with spleen preservation [(MD-273.10,95％ CI-354.39-191.081,P＜0.01),(OR 2.42,95％ CI 1.78-3.30,P＜0.01) respectively].The LDP group was significantly less than the ODP group in intraoperative blood loss,time to oral intake,and length of hospital stay [(MD-273.10,95％ CI -354.39-191.81,P＜0.01),(MD-1.78,95％ CI-2.36-1.20,P＜0.01),(MD-3.15,95％ CI-3.97-2.33,P＜0.01) respectively].There were no significant differences in blood transfusion,pancreatic fistula rate,and mortality between the two groups.Conclusions LDP is feasible and safe in treating pancreatic disease.When compared with ODP,LDP has the advantages of having less intraoperative blood loss,quicker recovery and more patients with spleen preservation.

FHIT and RASSF1A promoter hypermethylation in plasma and tumor tissues from patients with hepatocellular carcinoma / 中华肝胆外科杂志

Objective To detect aberrant methylation in the promoter of FHIT and RASSF1A genes in peripheral plasma and tumor tissues from patients with hepatocellular carcinoma (HCC) and to determine its clinical significance.Methods The methylation status of FHIT and RASSF1A genes in peripheral plasma and tumor tissues from 36 patients with HCC were detected by methylation-specific polymerase chain reaction(MSP).The correlation between methylation status in plasma and clinicopathological features was analyzed.Results The frequency of promoter methylation of FHIT in tissues was 75％ (27/36) and in plasma 52.8％ (19/36),and the correlation coefficient was r=0.482 (P=0.003).The frequency of promoter methylation of RASSF1A in tumor tissues was 83.3％ (30/36) and in plasma 61.1％ (22/36),and the correlation coefficient was r=0.561 (P=0.0004).Aberrant methylation of FHIT,RASSF1A gene in the plasma and tissues had no correlation with the patients' clinicopathological features such as gender,age,HBV/HCV infection,hepatic cirrhosis,tumor size,alpha-fetoprotein (AFP) level,pathological grade,staging,vascular tumour thrombus and recurrence.The sensitivity of AFP ≥400 μg/L was 44.4％,and AFP ≥20 μg/L 69.4％.The sensitivity of FHIT and RASSF1A gene promoter hypermethylation in 36 HCC patients was 72.2％.In 20 patients whose AFP ＜400 μg/L,the frequency of hypermethylation of the two genes together was 80％.When AFP ＜20 μg/L,the frequency of hypermethylation of the two genes together was 54.5 ％.Conclusions There was a significant concordance between plasma and tumor tissue methylation profiles.The methylation status in plasma and tumor tissues had no correlation with the patients' clinicopathological features.Combining promoter methylation of FHIT and RASSF1A genes was superior to AFP in the diagnosis of HCC.

Expression of chemokine VCC-1 in hepatocellular carcinoma / 中华肝胆外科杂志

Objective To investigate the expression of chemokine VCC-1 in hepatocellular carcinoma (HCC).Methods RT-PCR was used to detect the VCC-1 mRNA expressions in 8 HCC cell lines,10 normal liver tissues and 42 HCC tissues.Result In HCC cell lines,the expressions of VCC1 mRNA were high in SUN 398,intermediate in SUN387,SUN449,SUN423,HepG2,PLC5,and low in Hep3B and Huh7.In HCC tissues,the expressions of VCC-1 mRNA could be found in tumor and adjacent tissues.In these 42 tissues,VCC-1 mRNA was highly expressed in 26 specimens of tumor tissues (61％,14.9±7.6 fold) and 16 specimens of adjacent tissues (39％,6.9±5.4 fold).VCC-1 mRNA was up regulated in tumor tissues (P＜0.01).The expression levels of VCC-1 mRNA in 2 specimens were related to tumor differentiation and tumor size (P＜0.05).In the 10 specimens of normal liver tissues,no expression was detected in 8 specimens and light expression in 2 specimens.The expression was lower compared with cell lines,cancer tissues and adjacent tissues (P＜0.01).In 3 cases of recurrence,VCC-1 was highly expressed in cancer tissues (20.1±2.3 fold).In 8 specimenswith tumor thrombosis,5 tissues showed highly expressed VCC-1 (17.3±4.5 fold) while 3 specimens showed low expression.Conclusion VCC-1 plays an important role in HCC,and it may be considered as a potential therapeutic target of HCC.

Utilization of functional neck dissection for treatment of recurrent branchial cleft anomalies / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the utilization of functional neck dissection for treatment of recurrent branchial cleft anomalies.@*METHOD@#Fifteen patients with recurrent branchial cleft anomalies (fistula and cyst) undergoing functional neck dissection were retrospectively analyzed.@*RESULT@#Complications included 2 incisions secondary healing, 1 postoperative choking persisting for 1 months and 1 Horner's syndrome. There was no recurrence after a follow up from 2 months to 6 years,except 2 cases were lost to follow-up.@*CONCLUSION@#Functional neck dissection is an effective and safe surgical management for recurrent second and third branchial cleft anomalies(fistula and cyst).

Risk factors affecting early mortality and therapy of spontaneous rupture of hepatocellular carcinoma / 中国综合临床

Objective To investigate the risk factors affecting early mortality in spontaneous rupture in patients with hepatocellular carcinoma (HCC)and explore the effective treatment.Methed A retrospective study of 34 consecutive patients with spontaneous rupture of HCC from January 1998 to January 2008 was conducted.Followup was conducted for more than 3 years.18 clinical ,biochemical and pathological factors influencing 30-day mortality were analyzed.Results 30-day mortality rate was 38% (n=13).Univariate analysis showed that presence of cirrhosis,Child's C status,shock on admission,higher blood transfusion requirement,raised α-fetoprotein (AFP),raised alkaline phosphatase(ALP)and raised aspartate transaminase(AST)were all associated with increased risk early death (P＜0.05).Multivariate analysis displayed that shock was an independent risk factor affecting early death.The median survival time was 14.4 months in 8 patiens receiving emergency curative rescection and 44.4 months in 5 cases underwent delayed curative resection,and the 1-and 3-year survival rate were 63% (5/8)and 13% (1/8)in the former group and 80% (4/5)and 60% (3/5)in the latter group.Conclusion Poor prognosis of spontaneous rupture of HCC is associated with poor liver reservation,advanced disease and large volume of haemorrhage.Shock and blood transfusion requirement are the independent factors affecting early mortality.Curative resection is a definite treatment and delayed curative resection following initial haemostasis has a safer and more effective result.

Effects of lipoxygenase inhibitor NDGA on expression of 5-LOX and its apoptosis related genes in HepG2 cell line / 国际外科学杂志

Objective To investigate the effects of lipoxygenase inhibitor NDGA on expression of 5-LOX and its apoptosis related genes in HepG2 cell line.Methods The expression of 5-LOX and apoptosis related genes hTERT,bcl-2 and bax mRNA was determined by reverse transcriptasepolymerase chain reaction (RT-PCR).Results After 25,50,100,200 μmol/L NDGA treatment for24,48 h,the expression of 5-LOX of HepG2 cell decreased,but the expression of bax was up-regulated and the expressions of bcl-2 and hTERT mRNA were down-regulated,(P＜0.05 compared with the control group).The decrease in the expression of 5-LOX,hTERT and bcl-2 in HepG2 cell was negtively correlated with the dose duration of action of NDGA.Conclusion In vitro,5-LOX is expressed highly in HepG2 cell.Overexpression of 5-LOX may be related to the progression of hepatocellular carcinoma,NDGA can significantly decrease the expression of 5-LOX,up-regulate of bax and downregulation of bcl-2 and telomerse.Lipoxygenase might be a novel therapeutic target for the hepatocellular carinoma.

DNA methylation and gallbladder carcinoma / 国际外科学杂志

DNA methylation is a normal modification mode of eukaryon, there is an intimate relationship between aberrant promoter methylation of tumor-related genes and the generation or development of neo- plasms,emerging significantly biological effectiveness. Aberrant promoter methylation of tumor-related genes as the epigenetic markers, maybe play a very important role in the incidence, diagnosis, therapeutic effects, prognosis judgements and other aspects in gallbladder carcinoma.

Investigation of the surgical approach for resecting infratemporal fossa pterygomaxillary fossa-parapharyngeal space tumor / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the optimal surgical approach for resecting infratemporal fossa-pterygomaxillary fossa-parapharyngeal space tumor. The aim of this study is to enhance therapeutic effect and reduce complications and sequelae.@*METHOD@#Sixty-six patients with infratemporal fossa-pterygomaxillary fossa-parapharyngeal space tumor were analyzed retrospectively from 1998-2004, including complains, symptoms, physical signs; histodiagnosis, image examinations and surgical approaches.@*RESULT@#No recurrence was found in fifty benign tumor cases after 2 to 5 years, while in 16 cases with malignant tumor, four patients died in one year, 8 cases died in 2 to 4 years, and only 4 patients' survival time exceeded 4 years.@*CONCLUSION@#Extended maxillectomy is suitable for resecting primary carcinoma of maxillary sinus invading pterygomaxillary fossa, infratemporal fossa and /or parapharyngeal space tumor. also midface degloving approach is suitable for nasal primary cavity, nasal sinuses, nasopharynx and/or pterygomaxillary fossa tumor and localized malignant tumor. Trans-cervical combining mandibular split swing approach is suitable for parapharyngeal space tumor invading pterygomaxillary fossa and/or infratemporal fossa tumor. Trans-cervical jaw combining mandibulotomy is suitable for resecting parapharyngeal space, infratemporal fossa tumor for invading lateral skull base and pterygomaxillary fossa tumor.

Diagnosis and therapy of bronchial foreign body in aged patients / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the proper diagnosis and therapy method in aged patients with bronchial foreign body.@*METHOD@#A retrospective analysis was performed on 15 aged patients with bronchial foreign body by clinical characteristics. The experience of diagnosis and therapy in these cases were summarized.@*RESULT@#Misdiagnosed or missed diagnosis were found in 6 of 15 aged patients with bronchial foreign body. Three bronchial foreign bodies were taken out successfully under topical anesthesia. Twelve bronchial foreign bodies were taken out under topical anesthesia and balanced anesthesia. No complications happened in all case.@*CONCLUSION@#Clinical manifestation of bronchial foreign body is often unrepresentative in most aged patients. The chest X-ray of most cases can not show actual appearance of foreign bodies. In addition, history of foreign body in bronchus is often uncertain. So bronchial foreign bodies have often been misdiagnosed or missed diagnosed in aged patients. Suitable anesthesia method is the key to taking out bronchial foreign body in aged patients.